Human ANGPTL2/Angiopoietin-like 2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human ANGPTL2/Angiopoietin-like 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Thr260-His493.[Accession | Q9UKU9-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human ANGPTL2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human ANGPTL2 is greater than 95% as determined by SEC-HPLC.

SPR Data

Human LILRB2, hFc Tag captured on Protein A chip, can bind Human ANGPTL2, His Tag with an affinity constant of 0.14μM as determined in a SPR assay (Biacore T200).

背景(Background)

Angiopoietin-like 2 (ANGPTL2) is a proinflammatory protein belonging to the angiopoietin-like family. ANGPTL2 is secreted and detected in the systemic circulation. Different observational clinical studies reported that circulating levels of ANGPTL2 increase significantly in various chronic inflammatory diseases and showed associations between ANGPTL2 levels and diagnosis and/or prognosis of cardiovascular diseases, diabetes, chronic kidney disease, and various types of cancers.

分子别名(Synonyms)

ARP2; HARP; angiopoietin-like 2; ANGPTL2; ANGRP2; MGC8889

文献(References)

(1) Thorin-Trescases N, Thorin E. High Circulating Levels of ANGPTL2: Beyond a Clinical Marker of Systemic Inflammation. Oxid Med Cell Longev. 2017;2017:1096385. doi: 10.1155/2017/1096385. Epub 2017 Aug 24. PMID: 29138671; PMCID: PMC5613648.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.