货号价格

LIL-CM1A4-100μg / 询价

LIL-CM1A4-500μg / 询价

LIL-CM1A4-500μgx2 / 询价

Cynomolgus LILRA4/CD85g Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus LILRA4/CD85g Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu24-Asn446.[Accession | XP_005590346.1 ]

分子量大小(Molecular Weight)

The protein has a predicted MW of 47.7 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Cynomolgus LILRA4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus LILRA4 is greater than 95% as determined by SEC-HPLC.

背景(Background)

LILRA4, also known as ILT7 and CD85g, is an approximately 60-70 kDa variably glycosylated transmembrane protein that regulates immune cell activation. Mature human LILRA4 consists of a 423 amino acid (aa) extracellular domain (ECD) with four immunoglobulin-like domains, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain.LILRA4 function coreceptor to limit the innate immune responses to viral infections; signaling occurs via FCER1G (PubMed:16735691, PubMed:19564354). Down-regulates the production of IFNA1, IFNA2, IFNA4, IFNB1 and TNF by plasmacytoid dendritic cells that have been exposed to influenza virus or cytidine-phosphate-guanosine (CpG) dinucleotides, indicating it functions as negative regulator of TLR7 and TLR9 signaling cascades.

分子别名(Synonyms)

CD85g; ILT7; ILT-7; ILT7MGC129598; LILRA4; MGC129597

文献(References)

(1)Anderson K J , Allen R L. Regulation of T‐cell immunity by leucocyte immunoglobulin‐like receptors: innate immune receptors for self on antigen‐presenting cells[J]. Immunology, 2009, 127.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.