货号价格

LIL-HM4A5B-100μg / 询价

LIL-HM4A5B-500μg / 询价

LIL-HM4A5B-500μgx2 / 询价

Biotinylated Human LILRA5/CD85f/ILT11 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human LILRA5/CD85f/ILT11 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly42-Arg268.[Accession | A6NI73-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human LILRA5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human LILRA5 is greater than 95% as determined by SEC-HPLC.

背景(Background)

The leukocyte immunoglobulin-like receptors (LILR) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIRs). Human LILRA5, also known as ILT11, LIR-9, and CD85f, consists of a 227 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 10 aa cytoplasmic tail. LILRA5 may play a role in triggering innate immune responses. Does not seem to play a role for any class I MHC antigen recognition.

分子别名(Synonyms)

CD85; CD85f; ILT11; ILT-11; ILT11CD85f; LILRA5; LILRB7; LIR9; LIR-9

文献(References)

(1)LILRA5 is expressed by synovial tissue macrophages in rheumatoid arthritis, selectively induces pro-inflammatory cytokines and IL-10 and is regulated by TNF-alpha, IL-10 and IFN-gamma [J]. European Journal of Immunology, 2008, 38(12):3459-3473.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.