货号价格

LIL-HM4A6B-100μg / 询价

LIL-HM4A6B-500μg / 询价

LIL-HM4A6B-500μgx2 / 询价

Biotinylated Human LILRA6/CD85b/ILT8 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human LILRA6/CD85b/ILT8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly24-Asn447.[Accession | Q6PI73-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 49.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human LILRA6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human LILRA6 is greater than 95% as determined by SEC-HPLC.

背景(Background)

The LILRs are a family of receptors that regulate the activities of myelomonocytic cells. Specific allelic variants of two related members of the LILR family, LILRB3 and LILRA6, interact with a ligand exposed on necrotic glandular epithelial cells. The extracellular domains of LILRB3 and LILRA6 are very similar and their genes are highly polymorphic.

分子别名(Synonyms)

7M1; CD85b; ILT8; LILRA6; Pira3

文献(References)

(1) Jones DC, Hewitt CR, López-Álvarez MR, Jahnke M, Russell AI, Radjabova V, Trowsdale AR, Trowsdale J. Allele-specific recognition by LILRB3 and LILRA6 of a cytokeratin 8-associated ligand on necrotic glandular epithelial cells. Oncotarget. 2016 Mar 29;7(13):15618-31. doi: 10.18632/oncotarget.6905. PMID: 26769854; PMCID: PMC4941265.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.