货号价格

MHC-HM402-100μg / 询价

MHC-HM402-500μg / 询价

MHC-HM402-500μgx2 / 询价

Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA]

分子量大小(Molecular Weight)

The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.

背景(Background)

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and YLEPGPVTA peptide of  the gp100.

分子别名(Synonyms)

HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100

文献(References)

(1)Abad J D , Wrzensinski C , Overwijk W , et al. T-Cell Receptor Gene Therapy of Established Tumors in a Murine Melanoma Model[J]. Journal of Immunotherapy, 2008, 31(1):1-6.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.