BH7-HM174 | 价格

BH7-HM174-100μg / 询价

BH7-HM174-500μg / 询价

BH7-HM174-500μgx2 / 询价

Human B7-H4 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Phe29-Ala258 [Accession | Q7Z7D3-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.

纯度(Purity)

>95% as determined by Bis-Tris PAGE
>95% as determined by HPLC

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human B7-H4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human B7-H4 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human B7-H4, His Tag at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 3.1 ng/ml determined by ELISA.

背景(Background)

B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins. B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status .

分子别名(Synonyms)

B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291

文献(References)

(1) Smith J B, Caitlin S, Powell D J. B7-H4 as a potential target for immunotherapy for gynecologic cancers: a closer look[J]. Gynecologic Oncology, 2014, 134(1):181-189.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.