MHC多肽复合物
MHC（Major Histocompatibility Complex）为主要组织相容性复合体，是一类高度多态性的细胞表面蛋白家族，人类中也称HLA。MHC可与胞内抗原降解的肽段结合形成MHC多肽复合物，之后被运送到细胞表面，进而被相应T细胞受体TCR识别以启动免疫应答。
人类参与提呈抗原的MHC主要是MHC I和MHC II，MHC I结合抗原多肽后会被CD8+ T细胞识别，MHC II结合多肽后则被CD4+ T识别。MHC多肽复合物代表了一类胞内抗原靶点，其与TCR的独特结合模式，不仅在适应性免疫过程中发挥关键作用，也对TCR相关疗法如TCR-T开发具有重要意义。
Fig. 1 MHC参与抗原递呈的机理（Anthony W Purcell, et al., 2019）
恺佧生物提供一系列高品质MHC多肽复合物蛋白产品，可由哺乳动物细胞或大肠杆菌表达系统表达，涵盖NY-ESO-1、WT-1、MAGE-A、AFP和HPV16等热门靶点。同时，针对不同客户的特殊需求，恺佧生物特别提供TCR研究相关的定制服务，全力支持肿瘤免疫治疗药物研发。
产品优势
✔ 采用哺乳动物细胞或大肠杆菌表达系统表达，为研究者提供更多选择。
✔ 靶点种类丰富，涵盖NY-ESO-1、MAGE-A、WT-1、AFP等热门靶点。
✔ 产品形式多样，包括复合物单体、四聚体以及荧光标记等。
✔ 多种属覆盖，人、猴、鼠等不同种属。
✔ 质量可靠，均通过严格质检，确保优异的活性和稳定性。
✔ 提供TCR、TCR related drug和MHC多肽复合物表达等定制化服务
应用场景
✔ 动物免疫
✔ 抗体筛选
✔ 亲和力研究
✔ TCR筛选验证
✔ Blocking Assay
✔ SPR分析
数据示例
恺佧生物开发的MHC多肽复合物具备良好的生物学活性，在ELISA和SPR分析中表现优异。
Fig. 2 经ELISA验证，HLA-A*02:01&B2M&AFP (FMNKFIYEI)复合物可以结合Anti-AFP (HLA-A*02:01) Antibody，EC50为7.6 ng/ml。
Fig. 3 经SPR验证，HLA-A*02:01&B2M&AFP (FMNKFIYEI)复合物可与HLA-A*02:01&B2M&AFP TCR相结合，亲和力常数为0.923 μM。
恺佧生物开发的荧光标记的MHC多肽复合物四聚体在流式细胞水平分析中表现出优良的结合活性。
Fig. 4 经细胞水平流式检测，荧光标记的HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC)复合物四聚体可很好地与HLA-A*02:01&B2M&NY-ESO-1 TCR细胞结合。
恺佧生物提供TCR研究相关的专业定制服务，包括MHC多肽复合物、TCR及TCR related drug的表达。
以Tebentafusp (Kimmtrak)为例，恺佧生物成功表达出Tebentafusp，不仅可与HLA-A*02:01&B2M&GP100 (YLEPGPVTA)复合物结合，还可同时结合CD3异源二聚体，展示出良好的双向结合活性。数据示意：
Fig. 5 经ELISA验证，Tebentafusp可结合HLA-A*02:01&B2M&GP100 (YLEPGPVTA)复合物单体，EC50为11.8 ng/ml。
Fig. 6 在SPR分析中，Tebentafusp能与HLA-A*02:01&B2M&GP100 (YLEPGPVTA)复合物四聚体相结合，亲和力常数为0.196 nM。
Fig. 7 经ELISA验证，Tebentafusp可结合Human CD3E&CD3D，EC50值为8.7 ng/ml。
Fig. 8 经ELISA验证，Tebentafusp可结合Human CD3E&CD3G，EC50值为0.22 μg/ml。
产品列表
| Cat. No. | Protein Name | Peptide | Species | Tag | Exp. System | |
|---|---|---|---|---|---|---|
| MHC-HM446 | Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein |
SLLMWITQC |
Human | C-His-Avi | E.coli | 立即询价 |
| MHC-HM446T | Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein |
SLLMWITQC |
Human |
C-His-Avi |
E.coli |
立即询价 |
| MHC-HM405 | Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein | SLLMWITQC | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM405B | Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein |
SLLMWITQC |
Human |
C-His-Avi |
HEK293 |
立即询价 |
| MHC-HM405T | Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein | SLLMWITQC | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM418 | Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein | VVVGAVGVGK | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM418B | Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein |
VVVGAVGVGK |
Human |
C-His-Avi |
HEK293 |
立即询价 |
| MHC-HM418T | Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein | VVVGAVGVGK | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM420 | Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein | VVVGADGVGK | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM420B | Biotinylated Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein | VVVGADGVGK | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM420T |
Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer Protein |
VVVGADGVGK |
Human |
C-His-Avi |
HEK293 |
立即询价 |
| MHC-HM420TP |
PE-labeled Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Tetramer Protein |
VVVGADGVGK |
Human |
C-His-Avi |
HEK293 |
立即询价 |
| MHC-HM421 | Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein | VVVGAVGVGK | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM421B | Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein | VVVGAVGVGK | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM421F | FITC-labeled Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein |
VVVGAVGVGK |
Human |
C-His-Avi |
HEK293 |
立即询价 |
| MHC-HM421T | Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein |
VVVGAVGVGK |
Human |
C-His-Avi |
HEK293 |
立即询价 |
| MHC-HM421TP | PE-labeled Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein |
VVVGAVGVGK |
Human |
C-His-Avi |
HEK293 |
立即询价 |
| MHC-HM415 | Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein | HMTEVVRHC | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM415B | Biotinylated Human HLA-A*02:01&B2M&P53 R175H ( HMTEVVRHC) Monomer Protein | HMTEVVRHC | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM415T | Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer Protein | HMTEVVRHC | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM415TP | PE-labeled Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer Protein |
HMTEVVRHC |
Human |
C-His-Avi |
HEK293 |
立即询价 |
| MHC-HM416 | Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Monomer Protein |
HMTEVVRRC |
Human |
C-His-Avi |
HEK293 |
立即询价 |
| MHC-HM416B | Biotinylated Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Monomer Protein | HMTEVVRRC | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM416T | Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Tetramer Protein |
HMTEVVRRC |
Human |
C-His-Avi |
HEK293 |
立即询价 |
| MHC-HM416TP | PE-labeled Human HLA-A*02:01&B2M&P53 WT (HMTEVVRRC) Tetramer Protein |
HMTEVVRRC |
Human |
C-His-Avi |
HEK293 |
立即询价 |
| MHC-HM406T | Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Negative Control Protein | VMAPRTLVL | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM431 | Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein | RMFPNAPYL | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM431T | Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Tetramer Protein | RMFPNAPYL | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM402 | Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein | YLEPGPVTA | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM402T | Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein | YLEPGPVTA | Human | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM401 | Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein | GVYDGREHTV | Human | C-His-Avi | HEK293 | 立即询价 |
| HLG-HE41F | Human HLA-G Free Heavy Chain Protein | / | Human | C-His-Avi | E.coli | 立即询价 |
| HLG-HM41C | Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein | RIIPRHLQL | Human | C-His-Avi | HEK293 | 立即询价 |
| HLG-HM41CT | Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein | RIIPRHLQL | Human | C-His-Avi | HEK293 | 立即询价 |
| HLG-HM41CTB | Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein | RIIPRHLQL | Human | C-His-Avi | HEK293 | 立即询价 |
| HLG-RM41CT | Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein | RIIPRHLQL | Rhesus macaque | C-His-Avi | HEK293 | 立即询价 |
| HLG-CM41CT | Cynomolgus HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein | RIIPRHLQL | Cynomolgus | C-His-Avi | HEK293 | 立即询价 |
| MHC-HM413T | Human&Mouse Chimeric HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Tetramer Protein | CLGGLLTMV | Human | C-His-Avi | HEK293 | 立即询价 |
