货号价格

BSA-MM101-100μg / 询价

BSA-MM101-500μg / 询价

BSA-MM101-500μgx2 / 询价

Mouse Serum Albumin Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse Serum Albumin Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Glu25-Ala608.[Accession | P07724]

分子量大小(Molecular Weight)

The protein has a predicted MW of 70.19 kDa same as Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1 EU per ug by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3-6 months after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Mouse Serum Albumin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse Serum Albumin is greater than 95% as determined by SEC-HPLC.

SPR Data

Mouse Serum Albumin, His Tag immobilized on CM5 Chip can bind Mouse FcRn, His Tag with an affinity constant of 54.53 nM as determined in SPR assay (Biacore T200).

背景(Background)

Albumins are multifunctional proteins present in the blood serum of animals. They can bind and transport a wide variety of ligands which they accommodate due to their conformational flexibility. Serum albumins are highly conserved both in amino acid sequence and three-dimensional structure. Several mammalian and avian serum albumins (SAs) are also allergens. Sensitization to one of the SAs coupled with the high degree of conservation between SAs may result in cross-reactive antibodies in allergic individuals.

分子别名(Synonyms)

Albumin; ALB; Serum albumin; ANALBA; FDAH; PRO0883; PRO0903; PRO1341

文献(References)

(1) Chruszcz M, Mikolajczak K, Mank N, Majorek KA, Porebski PJ, Minor W. Serum albumins-unusual allergens. Biochim Biophys Acta. 2013 Dec;1830(12):5375-81. doi: 10.1016/j.bbagen.2013.06.016. Epub 2013 Jun 26. PMID: 23811341; PMCID: PMC4419372.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.