货号价格

CD3-HM157-100μg / 询价

CD3-HM157-500μg / 询价

CD3-HM157-500μgx2 / 询价

Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Asp23-Asp126 (CD3E) & Gln23-Ser116 (CD3G) [Accession | P07766(CD3E)&P09693(CD3G)].

分子量大小(Molecular Weight)

The protein has a predicted MW of 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.

纯度(Purity)

>95% as determined by Bis-Tris PAGE
>95% as determined by HPLC

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CD3E&CD3G, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2 ng/ml determined by ELISA (QC Test).

 

Immobilized Human CD3E&CD3G, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 36.2 ng/ml determined by ELISA.

SPR Data

Human CD3E&CD3G, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).

背景(Background)

T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E & CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

分子别名(Synonyms)

CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G

文献(References)

(1) Penaranda C , Tang Q , Bluestone J A. Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022.

(2)Gedeon P C , Schaller T H , Chitneni S K , et al. A rationally designed fully human EGFRvIII:CD3-targeted bispecific antibody redirects human T cells to treat patient-derived intracerebral malignant glioma.[J]. Clinical Cancer Research, 2018:clincanres.0126.2017.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.