货号价格

CD7-HM29B-100μg / 询价

CD7-HM29B-500μg / 询价

CD7-HM29B-500μgx2 / 询价

Human CD79B Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human CD79B Protein is expressed from HEK293 with hFc tag at the C-terminus.
It contains Ala29-Asp159 [Accession | P40259-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 41.9 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human CD79B on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD79B is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CD79B, hFc Tag at 1μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-CD79B Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test).

背景(Background)

CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta-chain) is a 36-40 kDa member of the Ig-Superfamily. It is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.

分子别名(Synonyms)

B29; CD79b molecule; CD79B; IGB; IGBAGM6; Ig-beta; Ig-β

文献(References)

(1) Dornan D , Bennett F , Chen Y , et al. Therapeutic potential of an anti-CD79b antibody-drug conjugate, anti-CD79b-vc-MMAE, for the treatment of non-Hodgkin lymphoma[J]. Blood, 2009, 114(13):2721-2729.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.