货号价格

CDE-CM201-100μg / 询价

CDE-CM201-500μg / 询价

CDE-CM201-500μgx2 / 询价

Cynomolgus CD3E/CD3 epsilon Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus CD3E/CD3 epsilon Protein is expressed from HEK293 with hFc tag at the C-Terminus.
It contains Gln22-Asp117 [Accession | Q95LI5-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Cynomolgus CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus CD3E is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Cynomolgus CD3E, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, hFc Tag with the EC50 of 14.9ng/ml determined by ELISA (QC Test).

背景(Background)

CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

分子别名(Synonyms)

CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18

文献(References)

(1)Karasu Ebru E, Nilsson Bo B, Köhl Jörg J, Lambris John D JD

(2)Front Immunol 2019-05-03 994 10

(3)Zelek Wioleta M WM, Taylor Philip R PR

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.