COV-VM1SM-100μg / 询价
COV-VM1SM-500μg / 询价
COV-VM1SM-500μgx2 / 询价
SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein
Recombinant SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685(N501Y,K417N,E484K) [Accession | YP_009724390.1].
The protein has a predicted MW of 75.9 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SARS-COV-2 Spike S1 (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike S1 (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.
Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K) , His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02μg/ml determined by ELISA.
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
(1)Belouzard S , Chu V C , Whittaker G R. Activation Of The Sars Coronavirus Spike Protein Via Sequential Proteolytic Cleavage At Two Distinct Sites[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(14):5871-5876.
