COV-VM2S1-100μg / 询价
COV-VM2S1-500μg / 询价
COV-VM2S1-500μgx2 / 询价
SARS-COV-2 Spike S1 Protein
Recombinant SARS-COV-2 Spike S1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val16-Arg685 [Accession | QHO60594.1].
The protein has a predicted MW of 101.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
Less than 1EU per μg by the LAL method.
Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human ACE2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA (QC Test).
Biotinylated Human ASGR1, His Tag captured on CM5 Chip via Anti-his antibody can bind SARS-COV-2 Spike S1, hFc Tag with an affinity constant of 0.132 μM as determined in SPR assay (Biacore T200).
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
(1)Ren W, Sun H, Gao GF, Chen J, Sun S, Zhao R, Gao G, Hu Y, Zhao G, Chen Y, Jin X, Fang F, Chen J, Wang Q, Gong S, Gao W, Sun Y, Su J, He A, Cheng X, Li M, Xia C, Li M, Sun L. Recombinant SARS-CoV-2 spike S1-Fc fusion protein induced high levels of neutralizing responses in nonhuman primates. Vaccine. 2020 Jul 31;38(35):5653-5658. doi: 10.1016/j.vaccine.2020.06.066. Epub 2020 Jun 24. PMID: 32651113; PMCID: PMC7311893.
