CXC-HM213 | 价格

CXC-HM213-100μg / 询价

CXC-HM213-500μg / 询价

CXC-HM213-500μgx2 / 询价

Human CXCL13/BCA-1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human CXCL13/BCA-1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.
It contains Val23-Per109 [Accession | O43927].

分子量大小(Molecular Weight)

The protein has a predicted MW of 37.6 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Bis-Tris PAGE result.

纯度(Purity)

>95% as determined by Bis-Tris PAGE
>95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human CXCL13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CXCL13/BCA-1 Protein is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CXCL13, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CXCL13 Antibody, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA (QC Test).

背景(Background)

Recent studies have implicated chemokines in microglial activation and pathogenesis of neuropathic pain. C-X-C motif chemokine 13 (CXCL13) is a B lymphocyte chemoattractant that activates CXCR5. Using the spinal nerve ligation (SNL) model of neuropathic pain, CXCL13 was persistently upregulated in spinal cord neurons after SNL, resulting in spinal astrocyte activation via CXCR5 in mice.

分子别名(Synonyms)

ANGIE; ANGIE2; BCA-1; BCA1; BLC; BLR1L; SCYB13

文献(References)

(1) Jiang BC, Cao DL, Zhang X, Zhang ZJ, He LN, Li CH, Zhang WW, Wu XB, Berta T, Ji RR, Gao YJ. CXCL13 drives spinal astrocyte activation and neuropathic pain via CXCR5. J Clin Invest. 2016 Feb;126(2):745-61. doi: 10.1172/JCI81950. Epub 2016 Jan 11. PMID: 26752644; PMCID: PMC4731172.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.