EGF-DE101-100μg / 询价
EGF-DE101-500μg / 询价
EGF-DE101-500μgx2 / 询价
Canine EGF Protein
Recombinant Canine EGF Protein is expressed from E.coli with His tag at the N-terminus.It contains Asn973-Arg1024 [Accession | Q9BEA0].
The protein has a predicted MW of 13.61 kDa. The protein migrates to 16-22 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Canine EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Canine EGF is greater than 95% as determined by SEC-HPLC.
Immobilized Canine EGF, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test).
Measured in a cell proliferation assay using Balb 3T3 mouse embryonic fibroblasts. The EC50 for this effect is typically 0.5 - 3 ng/mL.
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
HOMG4; URG
(1)Normanno N, Bianco C, De Luca A, Salomon DS. The role of EGF-related peptides in tumor growth. Front Biosci. 2001 May 1;6:D685-707. doi: 10.2741/normano. PMID: 11333208.
