货号价格

EGF-HM401-100μg / 询价

EGF-HM401-500μg / 询价

EGF-HM401-500μgx2 / 询价

Human EGFR/HER1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human EGFR/HER1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu25-Ser645.[Accession | P00533-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human EGFR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human EGFR is greater than 95% as determined by SEC-HPLC.

SPR Data

Human EGFR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human EGF, No Tag with an affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).

 

Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay (Biacore T200).

背景(Background)

The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

分子别名(Synonyms)

ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2

文献(References)

(1)Seshacharyulu P , Ponnusamy M P , Haridas D , et al. Targeting the EGFR signaling pathway in cancer therapy[J]. Expert Opinion on Therapeutic Targets, 2012.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.