货号价格

FCR-HM11B-100μg / 询价

FCR-HM11B-500μg / 询价

FCR-HM11B-500μgx2 / 询价

Human Fc gamma RIIIB/CD16b (NA1) Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Fc gamma RIIIB/CD16b (NA1) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly17-Ser200(NA1).[Accession | AAA35881.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 22.52 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human Fc gamma RIIIB (NA1) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Fc gamma RIIIB (NA1) is greater than 95% as determined by SEC-HPLC.

SPR Data

Human Fc gamma RIIIB (NA1), His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.55 μM as determined in SPR assay (Biacore T200).

背景(Background)

Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

分子别名(Synonyms)

CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10

文献(References)

(1)Qiu W Q , Bruin D D , Brownstein B H , et al. Organization of the human and mouse low-affinity Fc gamma R genes: duplication and recombination[J]. Science, 248.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.