货号价格

FER-HM101-100μg / 询价

FER-HM101-500μg / 询价

FER-HM101-500μgx2 / 询价

Human Fc epsilon RI alpha/FCER1a Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Val26-Gln205 [Accession | P12319-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per ug by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human Fc epsilon RI alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Fc epsilon RI alpha is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Fc epsilon RI alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.

背景(Background)

Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.

分子别名(Synonyms)

Fc-epsilon RI-alpha; FcERI; FCER1A; FCE1A

文献(References)

(1) Sanak M, Potaczek DP, Nizankowska-Mogilnicka E, Szczeklik A. Genetic variability of the high-affinity IgE receptor alpha subunit (Fc epsilon RI alpha) is related to total serum IgE levels in allergic subjects. Allergol Int. 2007 Dec;56(4):397-401. doi: 10.2332/allergolint.R-07-145. Epub 2007 Nov 1. PMID: 17965580.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.