货号价格

FGF-HM4ABB-100μg / 询价

FGF-HM4ABB-500μg / 询价

FGF-HM4ABB-500μgx2 / 询价

Biotinylated Human FGFR2 alpha (IIIb) Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human FGFR2 alpha (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg22-Glu378.[Accession | P21802-3]

分子量大小(Molecular Weight)

The protein has a predicted MW of 42.5 kDa. Due to glycosylation, the protein migrates to 70-110 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human FGFR2 alpha (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGFR2 alpha (IIIb) , His Tag with the EC50 of 20.3ng/ml determined by ELISA.

背景(Background)

Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs.

分子别名(Synonyms)

FGF R2a; FGFR2 alpha; KGFR; CD332; BBDS; BEK; BFR-1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25; FLJ98662

文献(References)

(1)Katoh, Masaru. FGFR inhibitors: Effects on cancer cells, tumor microenvironment and whole-body homeostasis (Review)[J]. International Journal of Molecular Medicine, 2016.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.