GDF-HM108-100μg / 询价
GDF-HM108-500μg / 询价
GDF-HM108-500μgx2 / 询价
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Human Latent GDF-8 Protein
Recombinant Human Latent GDF-8 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Asn24-Ser375 [Accession | O14793].
The protein has a predicted MW of 41.19 kDa. Due to glycosylation, the protein migrates to 35-40 kDa and 45-55 kDa based on Bis-Tris PAGE result.
>95% as determined by Bis-Tris PAGE
>95% as determined by HPLC
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Latent GDF-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Latent GDF-8 is greater than 95% as determined by SEC-HPLC.
Immobilized Human Latent GDF-8, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 22.8 ng/ml determined by ELISA (QC Test).
Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Myostatin; GDF8; Latent GDF8; Mstn
Walker RG, McCoy JC, Czepnik M, Mills MJ, Hagg A, Walton KL, Cotton TR, Hyvönen M, Lee RT, Gregorevic P, Harrison CA, Thompson TB. Molecular characterization of latent GDF8 reveals mechanisms of activation. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):E866-E875. doi: 10.1073/pnas.1714622115. Epub 2018 Jan 18. PMID: 29348202; PMCID: PMC5798348.
