Rat GDF15 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Rat GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.
It contains Ser189-Ala303 [Accession | Q9Z0J6].

分子量大小(Molecular Weight)

The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).

存储(Storage)

-20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Rat GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

ELISA Data

Immobilized Rat GDF-15, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Rat GDF15, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95μg/ml determined by ELISA.

背景(Background)

Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.

分子别名(Synonyms)

GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β

文献(References)

(1)Baek SJ, Eling T. Growth differentiation factor 15 (GDF15): A survival protein with therapeutic potential in metabolic diseases. Pharmacol Ther. 2019 Jun;198:46-58. doi: 10.1016/j.pharmthera.2019.02.008. Epub 2019 Feb 18. PMID: 30790643; PMCID: PMC7196666.