GPC-MM111-100μg / 询价
GPC-MM111-500μg / 询价
GPC-MM111-500μgx2 / 询价
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Mouse Glypican 1/GPC1 Protein
Recombinant Mouse Glypican 1/GPC1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp24-Ser529.[Accession | Q9QZF2]
The protein has a predicted MW of 56.8 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in 50mM Tris,150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse Glypican 1/GPC1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Glypican 1/GPC1 is greater than 95% as determined by SEC-HPLC.
CAR-T cells targeting glypican-1 (GPC1)-specific human and murine CAR-T cells generated from our original anti-human/mouse GPC1 antibody showed strong antitumor effects in xenogeneic and syngeneic mouse models, respectively. Importantly, the murine CAR-T cells enhanced endogenous T cell responses against a non-GPC1 tumor antigen through the mechanism of antigen-spreading and showed synergistic antitumor effects with anti-PD-1 antibody without any adverse effects in syngeneic models.
Glypican-1; FLJ38078; GPC1
(1) Kato D, Yaguchi T, Iwata T, Katoh Y, Morii K, Tsubota K, Takise Y, Tamiya M, Kamada H, Akiba H, Tsumoto K, Serada S, Naka T, Nishimura R, Nakagawa T, Kawakami Y. GPC1 specific CAR-T cells eradicate established solid tumor without adverse effects and synergize with anti-PD-1 Ab. Elife. 2020 Mar 31;9:e49392. doi: 10.7554/eLife.49392. PMID: 32228854; PMCID: PMC7108862.
