货号价格

IGG-HM004-100μg / 询价

IGG-HM004-500μg / 询价

IGG-HM004-500μgx2 / 询价

Human IgG4 Fc Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IgG4 Fc Protein is expressed from HEK293 without tag. It contains Glu99-Gly326.[Accession | P01861]

分子量大小(Molecular Weight)

The protein has a predicted MW of 25.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human IgG4 Fc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human IgG4 Fc is greater than 95% as determined by SEC-HPLC.

SPR Data

Human FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IgG4 Fc, No Tag with an affinity constant of 1.365 μM as determined in SPR assay (Biacore T200).

背景(Background)

It is known as a IgG4-related disease and its differentiation is based on the analysis of IgG4 levels in the affected tissues. The IgG4-related disease is considered to be a generalized pathological process involving a wide spectrum of various disorders that may affect distant organs.

分子别名(Synonyms)

IGHG4

文献(References)

(1)Touzani F, Pozdzik A. New insights into immune cells cross-talk during IgG4-related disease. Clin Immunol. 2019;198:1-10. doi:10.1016/j.clim.2018.11.004

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.