IHU-VM1RD-100μg / 询价
IHU-VM1RD-500μg / 询价
IHU-VM1RD-500μgx2 / 询价
SARS-Cov-2 Spike RBD (B.1.640.2/IHU) Protein
Recombinant SARS-Cov-2 Spike RBD(B.1.640.2/IHU) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(R346S, N394S, Y449N, E484K, F490S, N501Y) [Accession | QHD43416.1].
The protein has a predicted MW of 25.76 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SARS-Cov-2 Spike RBD (B.1.640.2/IHU) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-Cov-2 Spike RBD (B.1.640.2/IHU) is greater than 95% as determined by SEC-HPLC.
Immobilized SARS-Cov-2 Spike RBD (B.1.640.2/IHU) , His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 41.3ng/ml determined by ELISA.
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
S protein RBD; S glycoprotein RBD; Spike protein RBD
(1)Larry J Anderson, Ralph S Baric. Emerging Human Coronaviruses - Disease Potential and Preparedness[J]. N Engl J Med, 2012, 367(19):1850-1852.
