ILR-HM46RB | 价格

ILR-HM46RB-100μg / 询价

ILR-HM46RB-500μg / 询价

ILR-HM46RB-500μgx2 / 询价

Biotinylated Human IL-6 R alpha/CD126 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Leu20-Pro365 [Accession | P08887-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human IL-6 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human IL-6, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-6 R alpha, His Tag with the EC50 of 0.78μg/ml determined by ELISA.

背景(Background)

The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed.

分子别名(Synonyms)

IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; IL6R; CD126; IL6RQ; gp80

文献(References)

(1)Schmidt-Arras D , Rose-John S. IL-6 pathway in the liver: from physiopathology to therapy[J]. Journal of Hepatology, 2016:S0168827816000830.D

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.