KR3-HM4L3 | 价格

KR3-HM4L3-100μg / 询价

KR3-HM4L3-500μg / 询价

KR3-HM4L3-500μgx2 / 询价

Human KIR3DL3 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human KIR3DL3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Glu26-Leu322 [Accession | Q8N743].

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 0.2M L-arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human KIR3DL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human KIR3DL3 is greater than 95% as determined by SEC-HPLC.

SPR Data

Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200).

背景(Background)

Killer-cell immunoglobulin-like receptor (KIR) 3DL3 is a framework gene present in all human KIR haplotypes.KIR3DL3 has been shown to be constitutively expressed at a low RNA level in peripheral blood mononuclear cell (PBMC) and decidual natural kill (NK) cells.

分子别名(Synonyms)

CD158Z; KIR2DS2; KIR3DL7; KIR44; KIRC1; KIR3DL3

文献(References)

(1)Jones DC, Hiby SE, Moffett A, Trowsdale J, Young NT. Nature of allelic sequence polymorphism at the KIR3DL3 locus. Immunogenetics. 2006;58(8):614-627. doi:10.1007/s00251-006-0130-5

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.