MET-HM201-100μg / 询价
MET-HM201-500μg / 询价
MET-HM201-500μgx2 / 询价
Human HGF R/c-MET Protein
Recombinant Human HGF R/c-MET Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu25-Thr932 [Accession | P08581-1].
The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 95.9 kDa (β chain Fc chimera). Due to glycosylation, the protein migrates to 45-55 kDa and 100-120 kDa based on Bis-Tris PAGE result.
>95% as determined by Bis-Tris PAGE
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human HGF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human HGF, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9 ng/ml determined by ELISA (QC Test).
Immobilized Human HGF R, hFc Tag at 5 μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 26.7 ng/ml determined by ELISA.
c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling.
MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor
(1) Sierra J. c-MET as a potential therapeutic target and biomarker in cancer[J]. Therapeutic Advances in Medical Oncology, 2011, 3(1 Suppl):21-35.
