MFC-MM001-100μg / 询价
MFC-MM001-500μg / 询价
MFC-MM001-500μgx2 / 询价
Mouse IgG1 Fc Protein
Recombinant Mouse IgG1 Fc Protein is expressed from HEK293 without tag. It contains Val98-Lys324.[Accession | P01868]
The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse IgG1 Fc on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse IgG1 Fc is greater than 95% as determined by SEC-HPLC.
Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse IgG1 Fc, No Tag with an affinity constant of 15.09 nM as determined in SPR assay (Biacore T200).
Recombinant murine Fc fragment contains the hinge region, CH2, and CH3 region of the mouse IgG1 isotype. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.
IgG1; Igh-4; RGD1359539; VH7183
(1)Sockolosky J T , Szoka F C. The neonatal Fc receptor, FcRn, as a target for drug delivery and therapy[J]. Advanced Drug Delivery Reviews, 2015:S0169409X15000162.
