NEC-HM204 | 价格

NEC-HM204-100μg / 询价

NEC-HM204-500μg / 询价

NEC-HM204-500μgx2 / 询价

Human Nectin-4 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Nectin-4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.
It contains Gly32-Ser349 [Accession | Q96NY8].

分子量大小(Molecular Weight)

The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 72-74 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC

The purity of Human Nectin-4 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Nectin-4, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1μg/ml determined by ELISA (QC Test).

SPR Data

Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi Tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).

背景(Background)

Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.

分子别名(Synonyms)

EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4

文献(References)

(1)Challita-Eid P M , Satpayev D , Yang P , et al. Enfortumab Vedotin Antibody-Drug Conjugate Targeting Nectin-4 is a Highly Potent Therapeutic Agent in Multiple Preclinical Cancer Models[J]. Cancer Research, 2016:0008-5472.CAN-15-1313.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.