NEC-HM414B | 价格

NEC-HM414B-100μg / 询价

NEC-HM414B-500μg / 询价

NEC-HM414B-500μgx2 / 询价

Biotinylated Human Nectin-4 Protein IgV Domain

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human Nectin-4 Protein IgV Domain is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Gly32-Leu146 [Accession | Q96NY8-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 15.8 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human Nectin-4 IgV Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human Nectin-4 IgV Domain is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated Human Nectin-4 IgV Domain, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test).

背景(Background)

Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.

分子别名(Synonyms)

EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4

文献(References)

(1) Challita-Eid P M , Satpayev D , Yang P , et al. Enfortumab Vedotin Antibody-Drug Conjugate Targeting Nectin-4 is a Highly Potent Therapeutic Agent in Multiple Preclinical Cancer Models[J]. Cancer Research, 2016:0008-5472.CAN-15-1313.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.