NRP-HM101 | 价格

NRP-HM101-100μg / 询价

NRP-HM101-500μg / 询价

NRP-HM101-500μgx2 / 询价

Human Neuropilin-1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Neuropilin-1 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Phe22-Lys644 [Accession | NP_001019799.1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 70.97 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human Neuropilin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Neuropilin-1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human VEGF165, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 0.57μg/ml determined by ELISA.

背景(Background)

Neuropilin 1 (NRP1) is a transmembrane glycoprotein that acts as a co-receptor for a number of extracellular ligands including class III/IV semaphorins, certain isoforms of vascular endothelial growth factor and transforming growth factor beta.

分子别名(Synonyms)

BDCA4; NP1; Neuropilin-1; NRP1; CD304; VEGF165R; Neuropilin 1; NRP; Transmembrane Receptor; CD304 Antigen

文献(References)

Chaudhary B, Khaled YS, Ammori BJ, Elkord E. Neuropilin 1: function and therapeutic potential in cancer. Cancer Immunol Immunother. 2014 Feb;63(2):81-99. doi: 10.1007/s00262-013-1500-0. Epub 2013 Nov 22. PMID: 24263240.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.