PGF-MM201-100μg / 询价
PGF-MM201-500μg / 询价
PGF-MM201-500μgx2 / 询价
表达区间及表达系统(Source)
|
Recombinant Mouse PGF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val19-Pro158 [Accession | P49764]. |
分子量大小(Molecular Weight)
|
The protein has a predicted MW of 42.4 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
纯度(Purity)
|
> 95% as determined by Bis-Tris PAGE |
内毒素(Endotoxin)
|
Less than 1EU per μg by the LAL method. |
制剂(Formulation)
|
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution)
|
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储(Storage)
|
-20 to -80°C for 12 months as supplied from date of receipt. |
Recombinant Mouse PGF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val19-Pro158 [Accession | P49764].
The protein has a predicted MW of 42.4 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Placental growth factor (PGF) is another member of the VEGF family of cytokines with pro-angiogenic and pro-inflammatory effects. Retinal inhibition of PGF in combination with VEGF-A prevents vascular leakage and CNV possibly via modulating their own expression in mononuclear phagocytes. PGF-related, optimized strategies to target inflammation-mediated angiogenesis may help to increase efficacy and reduce non-responders in the treatment of wet AMD patients.
PlGF; PGF; PGFL; SHGC-10760; D12S1900; PlGF-2
(1) Balser C, Wolf A, Herb M, Langmann T. Co-inhibition of PGF and VEGF blocks their expression in mononuclear phagocytes and limits neovascularization and leakage in the murine retina. J Neuroinflammation. 2019 Feb 7;16(1):26. doi: 10.1186/s12974-019-1419-2. PMID: 30732627; PMCID: PMC6366121.
PGF-MM201-100μg / 询价
PGF-MM201-500μg / 询价
PGF-MM201-500μgx2 / 询价
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