RNK-HM211-100μg / 询价
RNK-HM211-500μg / 询价
RNK-HM211-500μgx2 / 询价
Human TNFRSF11A/Rank Protein
Recombinant Human TNFRSF11A/Rank Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile30-Pro212 [Accession | Q9Y6Q6-1].
The protein has a predicted MW of 46.85 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human TNFRSF11A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TNFRSF11A is greater than 95% as determined by SEC-HPLC.
Immobilized Human RANKL, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.
TNFRSF11A, also known as receptor activator of NF-κB (RANK), activates several signaling pathways, such as NF-κB, JNK, ERK, p38α, and Akt/PKB. RANK/TNFRSF11A is a novel and frequent target for de novo methylation in gliomas, which affects apoptotic activity and focus formation thereby contributing to the molecular pathogenesis of gliomas.
ODFR; Receptor activator of NF-KB; NF-KB; NFKB; CD265; TNFRSF11A; Rank; FEO; LOH18CR1; OPTB7; OSTS; PDB2; TRANCER;
von dem Knesebeck A, Felsberg J, Waha A, Hartmann W, Scheffler B, Glas M, Hammes J, Mikeska T, Yan PS, Endl E, Simon M, Reifenberger G, Pietsch T, Waha A. RANK (TNFRSF11A) is epigenetically inactivated and induces apoptosis in gliomas. Neoplasia. 2012 Jun;14(6):526-34. doi: 10.1596/neo.12360. PMID: 22787434; PMCID: PMC3394195.
