货号价格

SMF-HM407-100μg / 询价

SMF-HM407-500μg / 询价

SMF-HM407-500μgx2 / 询价

Human SLAMF7/CRACC/CD319 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human SLAMF7/CRACC/CD319 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser23-Met226.[Accession | Q9NQ25-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human SLAMF7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human SLAMF7 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human SLAMF7, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-SLAMF7 Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.

背景(Background)

CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 65-75 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family.Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response.

分子别名(Synonyms)

CD2 subset 1; CRACC; Novel Ly9; CD319; SLAMF7; CS1; 19A; FOAP-12

文献(References)

(1)Niels W. C. J. van de Donk, Moreau P, Plesner T, et al. Clinical efficacy and management of monoclonal antibodies targeting CD38 and SLAMF7 in multiple myeloma[J]. Blood, 2016, 127(6):681-695.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.