VGF-HM4R2B-100μg / 询价
VGF-HM4R2B-500μg / 询价
VGF-HM4R2B-500μgx2 / 询价
Biotinylated Human VEGF R2/KDR Protein
Recombinant Biotinylated Human VEGF R2/KDR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala20-Glu764 [Accession | P35968-1].
The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human VEGF R2 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human VEGF R2, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test).
Immobilized Human VEGF165, No Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, HisTag with the EC50 of 2.5μg/ml determined by ELISA.
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.
CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73
(1)Miettinen M , Rikala M S , Rys J , et al. Vascular Endothelial Growth Factor Receptor 2 as a Marker for Malignant Vascular Tumors and Mesothelioma:, An Immunohistochemical Study of 262 Vascular Endothelial and 1640 Nonvascular Tumors[J]. The American Journal of Surgical Pathology, 2012, 36(4):629-39.
