APN-MM101 | 价格

APN-MM101-100μg / 询价

APN-MM101-500μg / 询价

APN-MM101-500μgx2 / 询价

Mouse ANGPT2/Angiopoietin-2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Lys275-Phe496 [Accession | O35608].

分子量大小(Molecular Weight)

The protein has a predicted MW of 26.29 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse ANGPT2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse ANGPT2 is greater than 95% as determined by SEC-HPLC.

背景(Background)

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies..

分子别名(Synonyms)

AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand

文献(References)

(1)Wu X , Giobbie-Hurder A , Liao X , et al. Angiopoietin-2 as a Biomarker and Target for Immune Checkpoint Therapy[J]. Cancer Immunology Research, 2017, 5(1):17-28.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.