BAF-HM10R | 价格

BAF-HM10R-100μg / 询价

BAF-HM10R-500μg / 询价

BAF-HM10R-500μgx2 / 询价

Human BAFFR/TNFRSF13C Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Ser7-Ala71 [Accession | Q96RJ3-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 7.6 kDa. Due to glycosylation, the protein migrates to 13-25 kDa based on Bis-Tris PAGE result.

纯度(Purity)

>95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human BAFFR on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

ELISA Data

Immobilized Human BAFFR, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human BAFFR, His Tag at 0.5 μg/ml (100 μl/Well). Dose response curve for Anti-BAFFR Antibody, hFc Tag with the EC50 of 6.2 ng/ml determined by ELISA.

SPR Data

Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF Trimer, hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).

背景(Background)

BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF.

分子别名(Synonyms)

CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin

文献(References)

(1) Tin K , Peng C , Christopher T , et al. BAFF Receptor mAb Treatment Ameliorates Development and Progression of Atherosclerosis in Hyperlipidemic ApoE?/? Mice[J]. PLoS ONE, 2013, 8(4):e60430-.