Biotinylated Human BAFF/TNFSF13B/CD257 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.
It contains Ala134-Leu285 [Accession | Q9Y275-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 20.72 kDa. Due to glycosylation, the protein migrates to 22-27 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human BAFF is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human BAFFR, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human BCMA, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 6.6ng/ml determined by ELISA.

背景(Background)

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

分子别名(Synonyms)

BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1

文献(References)

(1)Bossen C , Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.