CAM-HM4EPB-100μg / 询价
CAM-HM4EPB-500μg / 询价
CAM-HM4EPB-500μgx2 / 询价
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Biotinylated Human EpCAM/TROP1 Protein
Recombinant Biotinylated Human EpCAM/TROP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Lys265 [Accession | P16422].
The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..
The purity of Biotinylated Human EpCAM is greater than 95% as determined by SEC-HPLC.
Immobilized Anti-EpCAM Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 21.1ng/ml determined by ELISA (QC Test).
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; ACSTD1; DIAR5; gp40; HNPCC8; MOC31; TACST-1; TACSTD1; TROP1; ESA; KSA; MIC18; MK1
(1) Pak M G , Shin D H , Lee C H , et al. Significance of EpCAM and TROP2 expression in non-small cell lung cancer[J]. World Journal of Surgical Oncology, 2012, 10(1):53
