Rhesus macaque CD155/PVR Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Rhesus macaque CD155/PVR Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Asp28-Asn343 [Accession | Q0MSE6-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.5 kDa. Due to glycosylation, the protein migrates to 46-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Rhesus macaque CD155/PVR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Rhesus macaque CD155/PVR is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Rhesus macaque CD155, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 67.6ng/ml determined by ELISA (QC Test).

SPR Data

Human DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 μM as determined in SPR assay (Biacore T200).

背景(Background)

CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.

分子别名(Synonyms)

CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946

文献(References)

(1)Yu X , Harden K , C Gonzalez L , et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.