TIG-HM110-100μg / 询价
TIG-HM110-500μg / 询价
TIG-HM110-500μgx2 / 询价
Human TIGIT Protein
Recombinant Human TIGIT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met22-Pro141 [Accession | Q495A1-1].
The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.
Immobilized Human TIGIT, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA (QC Test).
Immobilized Human TIGIT, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Serial dilutions of Anti-TIGIT Antibody were added into Human TIGIT, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.46μg/ml.
Human CD155, hFc Tag captured on Protein A chip, can bind Human TIGIT, His Tag with an affinity constant of 0.17μM as determined in a SPR assay (Biacore T200).
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
TIGIT; VSIG9; VSTM3; WUCAM
(1)Yu X , Harden K , C Gonzalez L , et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57.
