CD6-HM101-100μg / 询价
CD6-HM101-500μg / 询价
CD6-HM101-500μgx2 / 询价
Human CD6 Protein
Recombinant Human CD6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His18-Leu402 [Accession | NP_006716.3].
The protein has a predicted MW of 42.14 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human CD6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD6 is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 μM as determined in SPR assay (Biacore T200).
The cell surface glycoprotein CD6 is expressed on leukocytes and mediates T cell trafficking across endothelial cell barriers. CD6 is a lymphocyte surface co-receptor physically associated with the T-cell receptor (TCR)/CD3 complex at the center of the immunological synapse.
CD6; TP120; T12
Simões IT, Aranda F, Casadó-Llombart S, Velasco-de Andrés M, Català C, Álvarez P, Consuegra-Fernández M, Orta-Mascaró M, Merino R, Merino J, Alberola-Ila J, González-Aseguinolaza G, Carreras E, Martínez V, Lozano F. Multifaceted effects of soluble human CD6 in experimental cancer models. J Immunother Cancer. 2020 Mar;8(1):e000172. doi: 10.1136/jitc-2019-000172. PMID: 32217757; PMCID: PMC7174071.
