CLE-HM25A-100μg / 询价
CLE-HM25A-500μg / 询价
CLE-HM25A-500μgx2 / 询价
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Human MDL-1/CLEC5A Protein
Recombinant Human MDL-1/CLEC5A Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Tyr26-Lys188 [Accession | Q9NY25-1].
The protein has a predicted MW of 46 kDa. Due to glycosylation, the protein migrates to 63-70 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human MDL-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MDL-1 is greater than 95% as determined by SEC-HPLC.
Immobilized Human MDL-1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA.
In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality.
MDL-1; CLECSF5; MDL1; CLEC5A; DAP12-associating lectin 1
(1) Riesen M, Feyst I, Rattanavirotkul N, Ezcurra M, Tullet JM, Papatheodorou I, Ziehm M, Au C, Gilliat AF, Hellberg J, Thornton JM, Gems D. MDL-1, a growth- and tumor-suppressor, slows aging and prevents germline hyperplasia and hypertrophy in C. elegans. Aging (Albany NY). 2014 Feb;6(2):98-117. doi: 10.18632/aging.100638. PMID: 24531613; PMCID: PMC3969279.
