Mouse M-CSF/CSF-1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse M-CSF/CSF-1 Protein is expressed from HEK293 without tag.
It contains Lys33-Glu262 [Accession | P07141-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 25.98 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

>95% as determined by Bis-Tris PAGE
>95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse M-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse M-CSF is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Mouse M-CSF, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 1.69μg/ml determined by ELISA (QC Test).

背景(Background)

CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

分子别名(Synonyms)

CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim

文献(References)

(1)Cannarile M A , Weisser M , Jacob W , et al. Colony-stimulating factor 1 receptor (CSF1R) inhibitors in cancer therapy[J]. Journal for ImmunoTherapy of Cancer, 2017, 5(1):53.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.