货号价格

DDR-MM1R1-100μg / 询价

DDR-MM1R1-500μg / 询价

DDR-MM1R1-500μgx2 / 询价

Mouse DDR1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse DDR1 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Asp22-Ala415 [Accession | Q03146-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 44.9 kDa. Due to glycosylation, the protein migrates to 60-65 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse DDR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse DDR1 is greater than 95% as determined by SEC-HPLC.

背景(Background)

Discoidin domain receptor1 (DDR1) is a collagen activated receptor tyrosine kinase and an attractive anti-fibrotic target. Its expression is mainly limited to epithelial cells located in several organs including skin, kidney, liver and lung. DDR1's biology is elusive, with unknown downstream activation pathways; however, it may act as a mediator of the stromal-epithelial interaction, potentially controlling the activation state of the resident quiescent fibroblasts.

分子别名(Synonyms)

HGK2; MCK-10; CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; DDR1; CD167; EDDR1; PTK3; CD167a; DDR; EC 2.7.10; EC 2.7.10.1; ENTRK4

文献(References)

(1) Moll S, Desmoulière A, Moeller MJ, Pache JC, Badi L, Arcadu F, Richter H, Satz A, Uhles S, Cavalli A, Drawnel F, Scapozza L, Prunotto M. DDR1 role in fibrosis and its pharmacological targeting. Biochim Biophys Acta Mol Cell Res. 2019 Nov;1866(11):118474. doi: 10.1016/j.bbamcr.2019.04.004. Epub 2019 Apr 5. PMID: 30954571.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.