EGF-MM101-100μg / 询价
EGF-MM101-500μg / 询价
EGF-MM101-500μgx2 / 询价
Mouse EGFR/HER1 Protein
Recombinant Mouse EGFR/HER1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu25-Ser647 [Accession | Q01279].
The protein has a predicted MW of 70.40 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse EGFR is greater than 95% as determined by SEC-HPLC.
Mouse EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse EGF, hFc Tag with an affinity constant of 3.98 nM as determined in SPR assay (Biacore T200).
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
ErbB; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
(1)Seshacharyulu P , Ponnusamy M P , Haridas D , et al. Targeting the EGFR signaling pathway in cancer therapy[J]. Expert Opinion on Therapeutic Targets, 2012.
