GHR-HM101 | 价格

GHR-HM101-100μg / 询价

GHR-HM101-500μg / 询价

GHR-HM101-500μgx2 / 询价

Human GHR/Growth Hormone R Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human GHR/Growth Hormone R Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Ala27-Tyr264 [Accession | P10912-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 28.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human GHR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human GHR is greater than 95% as determined by SEC-HPLC.

背景(Background)

Pegvisomant, a growth hormone receptor (GHR) antagonist, is a well-known drug that was designed to treat acromegaly. However, recent studies have indicated that the GHR is a "moonlighting" protein that may exhibit dual functions based on its localization in the plasma membrane and nucleus.

分子别名(Synonyms)

GH receptor; GHBP; GHR; GHR/BP

文献(References)

(1) Lan H, Li W, Li R, Zheng X, Luo G. Endocytosis and Degradation of Pegvisomant and a Potential New Mechanism That Inhibits the Nuclear Translocation of GHR. J Clin Endocrinol Metab. 2019 Jun 1;104(6):1887-1899. doi: 10.1210/jc.2018-02063. PMID: 30602026.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.