货号价格

HER-MM102-100μg / 询价

HER-MM102-500μg / 询价

HER-MM102-500μgx2 / 询价

Mouse Her2/ErbB2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse Her2/ErbB2 Protein is expressed from HEK293 with His tag at the N-terminus.
It contains Thr23-Thr653 [Accession | P70424].

分子量大小(Molecular Weight)

The protein has a predicted MW of 70.74 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse Her2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse Her2 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Mouse Her2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HER2 Antibody, Rabbit Fc Tag with the EC50 of 33.3ng/ml determined by ELISA.

背景(Background)

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane.

分子别名(Synonyms)

CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; NGL; TKR1; ERBB2; NEU; ENV; ENVW; ERVWE1; HERV7Q; HERVW

文献(References)

(1)Tagliabue E , Balsari A , Campiglio M , et al. HER2 as a target for breast cancer therapy[J]. EXPERT OPINION ON BIOLOGICAL THERAPY, 2010, 10(5):711-724.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.