IGF-HM2BP | 价格

IGF-HM2BP-100μg / 询价

IGF-HM2BP-500μg / 询价

IGF-HM2BP-500μgx2 / 询价

Human IGFBP-7 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IGFBP-7 Protein Protein is expressed from HEK293 with His tag at the N-Terminus.
It contains Ser27-Leu282 [Accession | Q16270].

分子量大小(Molecular Weight)

The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human IGFBP-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human CD93, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IGFBP-7, hFc Tag with the EC50 of 0.69μg/ml determined by ELISA (QC Test).

背景(Background)

IGFBP-7, also known as Mac25/Angiomodulin (AGM), GFBP-rp1, tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide. IGFBP-7 binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.

分子别名(Synonyms)

IGFBP-7; IBP7; AGM; FSTL2; IGFBP-7v; IGFBPRP1; MAC25; PSF; RAMSVPS; TAF

文献(References)

(1)Li N, Han J, Tang J, Ying Y. IGFBP-7 inhibits the differentiation of oligodendrocyte precursor cells via regulation of Wnt/β-Catenin signaling. J Cell Biochem. 2018;119(6):4742-4750. doi:10.1002/jcb.26654.