IL6-CM001 | 价格

IL6-CM001-100μg / 询价

IL6-CM001-500μg / 询价

IL6-CM001-500μgx2 / 询价

Cynomolgus IL-6 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus IL-6 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Ala28-Met212 [Accession | P79341].

分子量大小(Molecular Weight)

The protein has a predicted MW of 22.06 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Cynomolgus IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus IL-6 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Cynomolgus IL-6, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.

背景(Background)

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.

分子别名(Synonyms)

IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF;IFN-β-2

文献(References)

(1)Gabay C. Interleukin-6 and chronic inflammation[J]. Arthritis Research & Therapy, 2006, 8.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.